How many Queens in one test tube

[Lorcan]

I had a messor aciculatus us colony which I moved into a hybrid nest 2.0 too soon and they didn't do well. I recently moved them back into a test tube and decided to boost the colony with three new queen's. 2 have joined one is resisting. I was wondering how many Queens is it advised to keep in one test tube.

[Johnathan]

Are they polygynous? If so as many as can comfortably fit.

[Lorcan]

Thanks for the reply they seem to be doing fine now.