Workers with polygynous queen

[HaydenP]

I’ve caught 2 pheidole Queens, and I have one in a test tube setup and one in a setup with workers. I didn’t put them both in the same setup in case one or the other didn’t work. If it is recommended, I can switch this around. The workers seem to be really cool with the one queen in there and I don’t know if two will mess up the chemistry. What should I feed the one with workers? And should I feed the queen that doesn’t have workers as well? I believe it was a colony formed by budding, and I really want to keep a pheidole colony. Any info would be great!

[Antloverhuman]

Pheidoli are fully claustral so the single queen should be left alone till she gets workers. The one with workers can be fed honey and little bit of protein once a week (unless you have put tons of workers in which case feed them twice every week