New queens and males?

[Spinzy]

I have not got to the stage yet where my colony is full of young queens do they try to have a nuptial flight in captivity?

[MESSOR]

Yes if your colony is mature enough it would start to produce alites.
But I dont think it is too common. Under certain conditions, if you had alites they would try to fly i guess. But i doubt it would be successful as the "False climate" in your nest, would not match with outside.
And if they mated inside the nest they would be in-breeding resulting in weak queens that would probably die.